Mycoplasma taxonomy studiedy electrophoresis of cell proteins.

Identification of Mycoplasma and other microorganisms by polyacrylamide-gel electrophoresis of cell proteins.

The proteins of Mycoplasma cells of various species produce highly reproducible and species-specific electrophoretic patterns in polyacrylamide gels containing 5 m urea and 35% acetic acid. These electrophoretic patterns can be used for the rapid identification and classification of Mycoplasma. Preliminary results indicate that this method may also be used for the identification and classificat...

Mycoplasma Agalactiae II- Immuno-Electrophoresis Patternes of M. agalactiae

Immunogens of Brucella Abortus S19 Identified By Two-Dimensional Gel Electrophoresis and Immunoblotting

Background: Lipopolysaccharides (LPSs) and several antigenic proteins of Brucella have been considered for preparation of diagnostic reagents and subunit vaccines. The objective of this study was to identify and compare immunogens of B. abortus S19 which induce humoral immune responses in human, goat and rabbit. Material and Methods: The bacterial whole cell extract was prepared in extraction b...

APPLICATION OF TWO-DIMENSIONAL ELECTROPHORESIS AND NIH 3T3 CELL TRANSFECTION ASSAY IN THE STUDY OF TUMOR-AS SOCIATED PROTEINS AND GENOMIC DNA TUMOROGENICITY IN MALIGNANT HUMAN ESOPHAGEAL SPECIMENS

Total protein and DNA extracted from histologically diagnosed normal nonmalignant and esophageal tumor tissues were used for analysis of polypeptides pattern by two-dimensional gel electrophoresis and DNA transforming activity in NIH 3T3 cell transfection assay, respectively. In comparison to normal tissues, eight polypeptides underwent down-regulation or disappeared, while seven polypeptid...

Polymerase Chain Reaction of Mgc2 and 16S rRNA Genes for Detection of Mycoplasma gallisepticum

Mycoplasmas are very small bacteria lacking cell walls that belong to various genera within the class Mollicutes, and also the smallest organisms that can live independently. They are able to cause serious and chronic disease because of some unique characteristics. Mycoplasma gallisepticum (MG) is an important avian pathogen causing significant economical losses within the poultry industry. The...